ABSTRACT
Objective To observe the expression of methylation related silent target 1 (TMS1),DNA methyltransferase 1 (DNM T1)and methyl CpG binding domain protein 2 (MBD2),and the methylation of the TMS1 gene promoter,in hepatocellular carcinoma (HCC),and to investigate the relationship between the three genes and the clinical diagnostic significance.Methods The protein expression of TMS1,MBD2 and DNMT1 in 48 cases of HCC tissues and 48 case of normal tissues were detected by immunohistochemistry (IHC);the methylation status of TMS1 gene in serum of 34 patients with HCC,26 patients with hepatitis B and 23 healthy controls were analyzed by methylation specific polymerase chain reaction (MSP).Results The posi-tive rates of TMS1 expression in HCC tissues and normal tissues were 26.08% and 97.92%,respectively;the positive rate of MBD2 expression were 18.75% and 80.00%,respectively;the positive rate of expression were 77.08 % and 32.25%,respectively.The positive rates of TMS1 and MBD2 expression in HCC tissues were significantly lower than those in the normal tissues,and DNM T1 expression was higer than that in the normal tissues,and the difference was statistically significant (P<0.05).The expression TMS1 and MBD2 were neg-atively correlated with DNMT1 (P< 0.05).TMS1 protein expression was associated with TNM stage and tumor differentiation but not associated with the age and gender (P<0.05).The methylation detection rates of TMS1 gene in HCC group,hepatitis B group and healthy group were 70.6%,50.0% and 0.0%,respective-ly,and the differences were statistically significant (P<0.05).The methylation detection rate of HCC group and hepatitis b group was obviously higher than the normal group,which aggravated with the increase of tumor grade and stage.Conclusion TMS1 hypermethylation may be an early event in the carcinogenesis of liver tissue,the abnormal expression of TMS1,MBD2 and DNM T1 proteins plays an important role in the oc-currence and the development of HCC,w hich can be used as new molecular markers for the early diagnosis and prognosis of HCC,and become the new targets for the treatment of HCC.
ABSTRACT
Objective To compare the immunostaining of human epidermal growth factor receptor-2(HER-2) among three different clones,and to compare with the results of fluorescence in situ hybridization(FISH) detection,to provide evidences for pathological department to select appropriate antibodies.Methods 268 cases of invasive breast cancer were examined by immunohistochemistry using antibodies for HER-2 of 3 different clones.The results were compared with those done by FISH.Results Immunostaining using antibody clone SP3 showed 3+ reactions in 66 cases;Immunostaining using antibody clone EP3 showed 3+ reactions in 53 cases,while using polyclonal HER-2 antibody,80 cases showed 3+ reactions.There was statistical significance between those groups using different clones(P<0.05).Consistent with the FISH results,the Kappa values are 0.76,0.67,and 0.56,respectively.Conclusion It suggests detection of HER-2 using immunohistochemistry should prioritize selecting monoclonal antibodies,especially clone SP3.
ABSTRACT
Purpose To detect the incidence rate, average age and the expression of hMLH-1 and hMSH-2 of sporadic colorectal carci-noma ( SCC) with Han and Uygur patients. Methods The expression of hMLH-1 and hMSH-2 was detected in SCC for 60 cases of Uygur and 196 cases of Han by immunohistochemical method, including 60 Uygur and Han cases normal colorectal mucosa ( NCM) . Results The positive rate of hMLH-1 and hMSH-2 proteins expression in the NCM was 100%. There was a marked difference in the positive rate of hMLH-1 in SCC between Han (93. 4%, 183/196)and Uygur (75%, 45/60) (P0. 05). The average age of Han and Uygur SCC patients were 65. 64 years, 57. 63 years, respectively, and Uygur SCC cases were more likely to be diagnosed at less 40 years old (P<0. 05). The positive rate of hMLH-1 and hMSH-2 expression in the tubular adenoma was 100%. The positive rate of hMLH-1 and hMSH-2 expression in the tubulovillous adenoma in Uygur and Han were 66. 7%( 2/3 ) and 66. 7%( 2/3 ) , and 74. 2%(23/31) and 90. 3%(28/31), respectively, significantly lower than those of tubular adenoma (P<0. 05). The expression of hMLH-1 was positively correlated with that of hMSH-2 in SCC in Han(rs =0. 737, P<0. 05). The expression of hMLH-1 was positive-ly correlated with that of hMSH-2 in SCC in Uygur(rs =0. 383, P<0. 05). There exists marked difference in the positive rate of hM-LH-1 and hMSH-2 among difference age groups (P<0. 05). Conclusion There is a certain loss of hMLH-1 and hMSH-2 expression in SCC in Han and Uygur Chinese, which is related to adenoma and age. The expression of hMLH-1 in SCC tissue among Uygur pa-tients is not resemble to those of Han patients. The average age of Uygur SCC patients is younger than Han, and the positive rate of hMLH-1 is higher. Combined detection of hMLH-1 and hMSH-2 proteins may be used for judging the severity and prognosis of SCC in Xinjiang, which helps improve patients’ treatment program and rationalize their choices.